Conventional PCR systems require users to set a predetermined number of cycles based on assay- and sample-specific factors. When performing single-cell sequencing experiments, this requires knowledge ...

In RNA-seq library preparation, selecting the appropriate number of PCR cycles is a critical balancing act to avoid overcycling.

Here we describe a simple and rapid method for assembly and PCR-based accurate synthesis (PAS) of long DNA sequences. The PAS protocol involves the following five steps: (i) design of the DNA sequence ...

We describe a highly multiplex PCR approach that can identify 10-fold more targets in current real-time PCR assays without additional enzymes or separate reactions. This single-step, single-tube, ...

Early in the pandemic, the message from governments of all levels was crystal clear: If you have symptoms, get a PCR test. While that still largely holds true, rules about who should get tested and ...

A video advocating for an airline that vows to resist any future COVID-19 restrictions such as requirements to be vaccinated, wear a face mask, or provide a negative COVID-19 test, contains false ...

SINGAPORE - Scientists here say that despite the recent rise in the number of rapid Covid-19 tests available on the market, conventional polymerase chain reaction (PCR) tests are likely to remain in ...

Gene expression experiments measure changes in RNA transcript levels to compare how genes are up- or down-regulated across ...

Add Yahoo as a preferred source to see more of our stories on Google. For any American wondering if they have Covid-19, there are two kinds of tests available: polymerase chain reaction (PCR) and ...

Every American traveling abroad needs to take an antigen test to return to the United States. But the start of the trip is often even more cumbersome, as many other countries (nearly every one I have ...